Evaluation of the sensitivities of DNA extraction and PCR methods for detection of Giardia duodenalis in stool specimens.

نویسندگان

  • Kwannan Nantavisai
  • Mathirut Mungthin
  • Peerapan Tan-ariya
  • Ram Rangsin
  • Tawee Naaglor
  • Saovanee Leelayoova
چکیده

Sensitivities of DNA extraction methods and PCR methods for Giardia duodenalis were evaluated. A combination of the most sensitive methods, i.e., FTA filter paper and a PCR protocol using RH11/RH4 and GiarF/GiarR primers, showed no significant differences compared to immunofluorescence assay in terms of their sensitivities and specificities.

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عنوان ژورنال:
  • Journal of clinical microbiology

دوره 45 2  شماره 

صفحات  -

تاریخ انتشار 2007